Coding

Part:BBa_K4606002:Design

Designed by: Baochao Li   Group: iGEM23_Nanjing-NFLS   (2023-09-14)


Firefly-luciferase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Firefly-luciferase is an ideal reporter method for gene detection. By inserting it into the tail of the target gene, the expression level of the target gene can be reflected according to the luciferase activity.

Source

The firefly-luciferase gene sequense is from the Photinus pyralis genome.

References

Chen IY, Gheysens O, Ray S, et al. Indirect imaging of cardiac-specific transgene expression using a bidirectional two-step transcriptional amplification strategy. Gene Ther. 2010;17(7):827-838. doi:10.1038/gt.2010.30